what is good for capture of biotinylated DNA? - (Dec/11/2007 )

I am using biotinylated primer to do PCR. I am not sure which way is good to capture the PCR product. Any sugesstion will be appreciated.

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PCR extraction column?
Ethanol percipitation?
Avidin or Streptavidin coated beads?

Umm... could you repeat the question. I am not sure what you what to do with the biotinylated PCR product

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Avidin beads would work well. If you want to isolate the product on a larger surface eg array ,avidin- or streptavidin-coated slides.

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I am trying to use the biotin-labeled primer to get specific fragment. When I use one set of primer L and E, I can get some non specific PCR product from only one single primer E, If I label the primer L with biotin, after bindng with Streptavidin coated tube or bead, I can remove the non-specific PCR products. I am trying to find a easy, cheap method for purifying the PCR products.

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gel purification should do. Load your PCR products into a gel, run the gel and then cut out the slab that contains the desired PCR product.

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That is impossible, they have same size, only several base pair difference

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which company is better, how about Dynabeads Streptavidin from Invitrogen?

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By capture do you mean immobilize on beads and then elute ssDNA? I tired Dynabeads from Promega and had very poor binding, and I know of several people who complain of similar poor efficiency with them. I've heard the Magnasphere ones are much better, but they are more expensive.

Ginger

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