stickiness of protein - (Nov/28/2007 )
I am doing some cell based functional assays with human MIP-1-alpha, which is a 7.8 kD protein and have encountered some 2 fold variability in activity. The problem seems to be sticky nature of the protein. I used polypropylene tips and assay plates throughout but have not tried using siliconised items. My buffer also contains 0.2% BSA. Can anybody help me out?
-ellie-
QUOTE (ellie @ Nov 28 2007, 08:15 AM)
I am doing some cell based functional assays with human MIP-1-alpha, which is a 7.8 kD protein and have encountered some 2 fold variability in activity. The problem seems to be sticky nature of the protein. I used polypropylene tips and assay plates throughout but have not tried using siliconised items. My buffer also contains 0.2% BSA. Can anybody help me out?
plastic ware like cups can be silanized, to buy or self-made; tips to silanize is critical as it may have effect on accuracy of pipetted volume;
addition of 0.01 % triton x-100 may reduce stickyness
-The Bearer-