ÇóÖúmsp - (Jun/14/2004 )

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Procedure:
1. Add 5 volumes of 6M NaI to DNA solution or agarose gel slice
o if gel slice, melt at 55 degrees for 5 minutes with occasional agitation
o for aqueous solutions add 1/10 vol 3M NaOAc to ensure pH is less than 7
2. Bind DNA to silica
o add appropriate volume of silica suspension (binds approx 200 ng of DNA / ul of suspension) and mix well
o sediment silica matrix by centrifugation (10 seconds in 'nanofuge' or brief spin at full spend in microfuge)
o wash silica 3 times with 500 ul wash solution
o pellet silica once more to remove any residual wash solution
o air dry to remove any residual EtOH
3. Elute DNA from silica
o resuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer for downstream digests
o heat 5 minutes at 60 degrees
o spin 2 min @ full speed in microcentrifuge
o remove DNA-containing supernatant to clean tube
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