Identifying Stem Cells - which method (Nov/11/2007 )

Hello everybody

I would like to know which method I can use to identify stem cells in a sample of human tissue.
Is there a group of genes that are expressed only in undifferentiated cells?
There is a good label or a panel of good markers?

Thank you

EAG

-EAG-

CD34+CD38-

with FACS flow cytometry?

do you want stain your cells in whole blood?
Please look at Miltenyi Biotec websites

-creed-

QUOTE (creed @ Nov 12 2007, 10:08 AM)

CD34+CD38-

with FACS flow cytometry?

do you want stain your cells in whole blood?
Please look at Miltenyi Biotec websites

The ideia is look this cells in fine needle aspiration biopsy of thyroid tumor.

Thanks

EAG

-EAG-

I guess all stem cells share one characteristic -side population. If you have a flow cytometry with violet laser, you can stain with hoechest , the side population should be stem cells.

QUOTE (EAG @ Nov 12 2007, 04:11 PM)

QUOTE (creed @ Nov 12 2007, 10:08 AM)

CD34+CD38-

with FACS flow cytometry?

do you want stain your cells in whole blood?
Please look at Miltenyi Biotec websites

The ideia is look this cells in fine needle aspiration biopsy of thyroid tumor.

Thanks

EAG

-flakie-

Flakie,

Where can I learn more about the method that you suggested?

Any help would be great!

Thank you already for the attention and time.
Best regards,

EAG

QUOTE (flakie @ Nov 14 2007, 06:56 PM)

I guess all stem cells share one characteristic -side population. If you have a flow cytometry with violet laser, you can stain with hoechest , the side population should be stem cells.

QUOTE (EAG @ Nov 12 2007, 04:11 PM)

QUOTE (creed @ Nov 12 2007, 10:08 AM)

CD34+CD38-

with FACS flow cytometry?

do you want stain your cells in whole blood?
Please look at Miltenyi Biotec websites

The ideia is look this cells in fine needle aspiration biopsy of thyroid tumor.

Thanks

EAG

-EAG-