Why should some antibodies not be aliquoted? - (Oct/31/2007 )
QUOTE (Dominic @ Oct 31 2007, 03:11 PM)
i agree with missele - you only really need to aliquot if you are avoiding freeze thaw cycles, but you said fridge not freezer so you should be ok leaving it in the origional bottle - i doubt this is the cause of your weird results.
dom
dom
oops sorry,, i mean i keep it in the freezer since the supplier says "Store at -20 and do not aliquot".
(somehow i keep mixing up fridge and freezer
) -chrismartin-
does it go solid in the freezer? if so i would aliquot regardless
dom
-Dominic-
QUOTE (Dominic @ Nov 7 2007, 11:54 AM)
does it go solid in the freezer? if so i would aliquot regardless
dom
dom
it contains 50% glycerol,, and yup it becomes solid. on average the temperature of the freezer i use is -24 degrees.
in the end i aliquoted the Ab despite the datasheet info / critics I received from my bosses.
hope it helps.
-chrismartin-