How to analyze the result from TargetP? - (Oct/28/2007 )
I used the “TargetP (http://www.cbs.dtu.dk/services/TargetP/)” to analyze the protein, and the score was as follow:
Name Len cTP mTP SP other Loc RC TPlen
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***** 277 0.015 0.083 0.865 0.041 S 2 29
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cutoff 0.000 0.000 0.000 0.000
Does it mean that the protein is a secreted protein?
How can I prove it by experimental method?
If I did the Green fluorescence fusion in bombarded onion epidermal cells, it will be no signal since it’s a secreted protein, right?
Thank you in advance!
Name Len cTP mTP SP other Loc RC TPlen
--------------------------------------------------------------------------------------------------------------
***** 277 0.015 0.083 0.865 0.041 S 2 29
-----------------------------------------------------------------------------------------------------------------
cutoff 0.000 0.000 0.000 0.000
Does it mean that the protein is a secreted protein?
How can I prove it by experimental method?
If I did the Green fluorescence fusion in bombarded onion epidermal cells, it will be no signal since it’s a secreted protein, right?
Thank you in advance!
The analysis shows that the protein has a signal peptide (SP), its location is the secretory pathway, RC is the reliability class. There are 5 reliability classes (1-5). The smaller the value, the safer the prediction. TPlen is the predicted presequence length (29 aa).
You can find these information below your results ("EXPLAIN").