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Do you have protocol for isolation of RNA from seed? - (Oct/08/2007 )

HI! anyone have protocol for isolation of RNA from seed?..advanced thanks.

-neel-

What kind of seed?

What method are you using? Kit, trizol...
Some manufacturers have instructions for that kind of stuff. Do check that.

-Ambrósio-

QUOTE (Ambrósio @ Oct 8 2007, 07:30 PM)
What kind of seed?

What method are you using? Kit, trizol...
Some manufacturers have instructions for that kind of stuff. Do check that.


thanks...its rice seed and i have to use trizol

-neel-

Well, we work with rice here in the lab, but we only extract RNA from grown plants...

I've never tried doing so with seeds and I don't think anyone here in the lab has done so...

Sorry for not being much help...

-Ambrósio-