cDNA quantification using Nanodrop - (Sep/24/2007 )

Hi,

I don't have a lot of experience using Nanodrop in quantifying my RNA/DNA, but I don't have a lot of problem with it until I tried to quantify cDNA.

So... can you quantify cDNA using nanodrop?

-EPrincess-

well treat RNAse and purify to eliminaite remaining RNA and nucleotides, then measure with nanodrop. Ncleotides drives very bad measuremnets.

-fred_33-

QUOTE (fred_33 @ Sep 24 2007, 06:27 PM)

well treat RNAse and purify to eliminaite remaining RNA and nucleotides, then measure with nanodrop. Ncleotides drives very bad measuremnets.

Thank you for that! I had that idea before, but noone in my lab agreed that it is feasible. I'll give it a go, though!

-EPrincess-

I´ve always thought that it´s impossible to measure cDNA, at least you can´t be sure of your measurments?! Isn´t it so?!

-Dione-

QUOTE (Dione @ Oct 4 2007, 02:34 AM)

I´ve always thought that it´s impossible to measure cDNA, at least you can´t be sure of your measurments?! Isn´t it so?!

Why would it be impossible?

I thought I measured my cDNA once... so the concentration I got then is wrong?

-Ammie-

Be sure to choose the right kind of nucleic acid you are trying to measure. Somewhere on the screen there is a box for this. I can't remember offhand all the selections available, but I think single-stranded DNA is one of them.

-wbla3335-