stability of antibiotics. - (Sep/21/2007 )
can anyone tell me for how long ampicillin, kanamycin, spectinomycin, streptomycin and hygromycin are stable in LB-agar plates? what is the optimal temperature of their storage?
-bilaljilani-
QUOTE (bilaljilani @ Sep 21 2007, 05:42 AM)
can anyone tell me for how long ampicillin, kanamycin, spectinomycin, streptomycin and hygromycin are stable in LB-agar plates? what is the optimal temperature of their storage?
there is decline of antibiotics efficacy over the time (weeks);
after some weeks it is difficult to estimate the effective concentration; better use the plates freshly poured, or store plates without antibiotics and add them freshly before use; in this case you should use a fixed volume (f.i 20 ml of agar) to calculate what you have to add
-The Bearer-