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How much DNA probe is needed for Northern? - (Sep/17/2007 )

Hi, I have labelled my probe with North2South kit from Pierce and am about to start hybridization. Can someone suggest to me how much probe to use?

Pls!!!

-kenzo-

i use to label 10pmol DNA oligo for northern blot.
for random priming, i use 25ng template

-fred_33-