Switching MDA from L-15 medium - RPMI, DMEM, or something else? (Sep/07/2007 )

I recently purchased some MDA-MB-231 from ATCC, which is kept and grown in L-15 media. Our lab only has a CO2 incubator, due to other cell lines we use, and this obviously won't work well with the L-15 media, which requires 100% atmospehere. I emailed ATCC and they said to seal the flasks before putting them in the incubator and they'll be fine. Problem is, I need to be able to plate these cells and let them grow for the assay I want to do. As such, I want to switch the cells to another, bicarbonate-buffered medium, and I've seen both DMEM and RPMI batted about here and in the literature as possible replacements.

I've already looked up the formulation lists for L-15, DMEM, and RPMI - the RPMI has more types of components compared to the other two, but the DMEM tends to have more of the components it has. Beyond that, I've only found a post here saying that DMEM should be used for adherent cell lines, while RPMI for suspended lines. Besides this, are there any other factors to consider when switching media that I should be aware of?

Thanks for the help.

hi... I culture mda231s in my lab and use RPMI supplemented with P/S, 10% FBS, 1.25% L-Glut, and 1% sodium pyruvate. Thats just our standard media formulation that works with most of our current cell lines. Ive never had a problem using this media with them. hope that helps.