How to convert picomole to microgramme? - (Sep/07/2007 )
QUOTE (fred_33 @ Sep 12 2007, 07:44 PM)
well 100pmol in 10µL makes you have 1,716µg of DNA.
as it's quite few, basically i would dilute your stock solution by 2, and take take 1,16µl of this solution
as it's quite few, basically i would dilute your stock solution by 2, and take take 1,16µl of this solution
How do you calculate it? Can show me the way? And quite confuse when you say you would dilute the stock solution by 2. Thanks for the help. 
-cheerioet83-
the formula is in biomath calculator.
Diluting solution by 2 gives you a stock solution at ~0.85µg/µl which allows more accuracy in pipetting 1µg
-fred_33-
Concentration in micromoles * MW(g/mol) * 10^-6 (mol/micromoles) = micrograms/microliter
-beccaf22-
QUOTE (beccaf22 @ Sep 13 2007, 10:50 PM)
Concentration in micromoles * MW(g/mol) * 10^-6 (mol/micromoles) = micrograms/microliter
Thanks for it..I will try it out.
-cheerioet83-
QUOTE (fred_33 @ Sep 13 2007, 08:37 PM)
the formula is in biomath calculator.
Diluting solution by 2 gives you a stock solution at ~0.85µg/µl which allows more accuracy in pipetting 1µg
Diluting solution by 2 gives you a stock solution at ~0.85µg/µl which allows more accuracy in pipetting 1µg
Oh..I did go check back at the biomath calculator. Thanks. -cheerioet83-