protocol wanted - a protocol for pyronin and hoechst 33342 doual straing (Aug/24/2007 )
hi everyone i want to staing cell by two dyes: pyronin for rna and hoechst 33342 for dna, is there any protocol for that, or any clues no it, thanks advance.
-subberry-
QUOTE (subberry @ Aug 24 2007, 10:26 PM)
hi everyone i want to staing cell by two dyes: pyronin for rna and hoechst 33342 for dna, is there any protocol for that, or any clues no it, thanks advance.
Hoechst is a passive dye. You can add it straight into the media and incubate it for 5-15 min at 37 and then fix or visualize. Pretty simple.
7's
-Lenti77-
QUOTE (Lenti77 @ Aug 31 2007, 10:12 AM)
QUOTE (subberry @ Aug 24 2007, 10:26 PM)
hi everyone i want to staing cell by two dyes: pyronin for rna and hoechst 33342 for dna, is there any protocol for that, or any clues no it, thanks advance.
Hoechst is a passive dye. You can add it straight into the media and incubate it for 5-15 min at 37 and then fix or visualize. Pretty simple.
7's
what is a passive dye? If a dye is passive, it just incubation ok?is fixation needed？ And how about PY?
-subberry-