PCR product purification - (Apr/01/2004 )
I'm very satisfied with the UltraClean™ 15 DNA Purification Kit from Mobio.
Why don't you try with Agencourt Ampure Beckman Coulter, it's a nice method, easy to use and fast. With amplification of 500bp you won't have any problem... try it, I love it...
I also use UltraClean15 DNA purification Kit, and it is generally very good, and reasonably priced- with the added bonus that the same kit can be used for DNA extraction. I have been told that it is not as effective if you have a high number of primer dimers. Have also used Sephadex to clean PCRs in a time pressed situation which worked well, although i have not used it very often.
Although i personally have not had a problem with UltraClean15 DNA purification Kit, some other people i work with have. Before cleaning the PCR product is a clear band, but afterwards two smaller bands were visible (like it had been cut in some way). She sequenced from both directions. The sequences using the forward primer did not work but the reverse worked perfectly... we rang the company, but they couldn't help us (although they did send some replacement components of the kit).
I was wondering if anyone knew the explanation?
I successfully use the HighPure kits from Rochê. They are highest quality AND cheaper than Qiagen.
E.g. High Pure PCR Cleanup Micro Kit
They have a binding enhancer so that you can determine the product size you want to purify and which you don't (e.g. primer dimers). Elimantes even running always agarose gels once you are used to it 
Works great...
that has always worked wonderfully without giving any problems. ever tried that?
After purification (etoh pecipitation) of the PCR product from the agarose gel, for how many days does he PCR product will be in good state if sequencing of the PCR product is to be carried out
I've been using this Agencourt SPRI bead purification and it is fast...especially when doing 96 well plates...but I, and some of my colleagues, have had problems where much of the dna is lost after purification. Are there any tips or tricks anyone can suggest to not lose so much dna? Or any alternatives? I was thinking that maybe it's possible to use a robot for this...because when you use multichannel pipettes, the tips aren't always straight, and trying to avoid scraping the ring of beads while on the magnetic plate gets a bit tricky.
Anyone with experience in this?
Thanks.