How to Concentrate Under Reduced Pressure - (Aug/08/2007 )
This may be a fairly stupid question, but how does one concentrate something under reduced pressure? I am synthesizing NHS-folate by reacting folic acid, NHS, DCC and DMSO. Then filtering the dicyclohexylurea by-product. The protocol then says to concentrate the dimethyl sulfoxide solution under reduced pressure and heating, and finally to precipitate the NHS-folate in diethylether.
What does it mean to concentrate something under reduced pressure/heating?
-Serrrge-
QUOTE (Serrrge @ Aug 8 2007, 03:56 PM)
This may be a fairly stupid question, but how does one concentrate something under reduced pressure? I am synthesizing NHS-folate by reacting folic acid, NHS, DCC and DMSO. Then filtering the dicyclohexylurea by-product. The protocol then says to concentrate the dimethyl sulfoxide solution under reduced pressure and heating, and finally to precipitate the NHS-folate in diethylether.
What does it mean to concentrate something under reduced pressure/heating?
What does it mean to concentrate something under reduced pressure/heating?
This means you have to attach a pump (with a cold trap) to a distillation system so you can remove solvent at a temperature below its normal boiling point (this is especially important if you have something as unstable as NHS-folate). This is normally done in an apparatus known as rotary evaporator (rotavap), but as I said, if you don't have one, you can always set up a distillation system connected to a vacuum pump.
-ippys-