DMSO soluble drug when added to DMEM get clotted, Why - Very urgent!!!!!!!! (Aug/08/2007 )
Hi all,
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
-pBS-
QUOTE (pBS @ Aug 8 2007, 11:56 AM)
Hi all,
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
your agent is soluble in DMSO but not in water-based solution, so it precipitates when DMSO-solved agent is added to medium (dilution of DMSO); if your medium contains serum. you may add your DMSO-solved agent to stock serum in little portions, and looks what happens; may be better solubility of kaemperol by binding to plasma proteins; then added serum-solved agent to medium
-The Bearer-
Thanx for your kind response
I have to calculate the antiproliferative potential of kaempferol on the cancerous cells. would you please suggest me some suitable solvent i should use for this chemical.
i will be very much thankful.
QUOTE (The Bearer @ Aug 8 2007, 03:05 AM)
QUOTE (pBS @ Aug 8 2007, 11:56 AM)
Hi all,
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
your agent is soluble in DMSO but not in water-based solution, so it precipitates when DMSO-solved agent is added to medium (dilution of DMSO); if your medium contains serum. you may add your DMSO-solved agent to stock serum in little portions, and looks what happens; may be better solubility of kaemperol by binding to plasma proteins; then added serum-solved agent to medium
-pBS-
QUOTE (pBS @ Aug 8 2007, 01:31 PM)
Thanx for your kind response
I have to calculate the antiproliferative potential of kaempferol on the cancerous cells. would you please suggest me some suitable solvent i should use for this chemical.
i will be very much thankful.
your agent is soluble in DMSO but not in water-based solution, so it precipitates when DMSO-solved agent is added to medium (dilution of DMSO); if your medium contains serum. you may add your DMSO-solved agent to stock serum in little portions, and looks what happens; may be better solubility of kaemperol by binding to plasma proteins; then added serum-solved agent to medium
I have to calculate the antiproliferative potential of kaempferol on the cancerous cells. would you please suggest me some suitable solvent i should use for this chemical.
i will be very much thankful.
QUOTE (The Bearer @ Aug 8 2007, 03:05 AM)
QUOTE (pBS @ Aug 8 2007, 11:56 AM)
Hi all,
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
I have purchased kaempferol( Flavonoid, chemopreventive agent) from Sigma-aldrich Pvt Ltd. I dissolved it in DMSO( 100 %), problem arise when i added this to DMEM then it get clotted and get turbid.
i dont know why is this happening. if somebody have any solution or suggestion then kindly please me.
thanx for kind cooperation.
your agent is soluble in DMSO but not in water-based solution, so it precipitates when DMSO-solved agent is added to medium (dilution of DMSO); if your medium contains serum. you may add your DMSO-solved agent to stock serum in little portions, and looks what happens; may be better solubility of kaemperol by binding to plasma proteins; then added serum-solved agent to medium
follow the instructions of the provider if there is any; an alternative solvent should be ethanol; solubility is not endless but in a range of 10 -20 mg/ml
-The Bearer-