quantification of HCA - (Jul/30/2007 )

HAI FRIENDS
IS THERE ANY SIMPLE PROTOCOLS FOR THE QUANTIFICATION OF HCA(HYDROXY CITRIC ACID)
FROM THE FRUIT RIND OF GARCINIA BY HPLC OR ANY OTHER METHODS EXCLUDING TITRATION. IF AN'BODY HAS THIS IT IS VERY HELPFUL THANKYOU

-Asish.G.R-

hi.. check out this referecne.. DETERMINATION OF (-) HYDROXYCITRIC ACID IN COMMERCIAL SAMPLES OF GARCINIA CAMBOGIA EXTRACT BY LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION .. Journal of Liquid Chromatography & Related Technologies, Volume 23, Issue 6 March 2000 , pages 915 - 923

-lokesh-

QUOTE (lokesh @ Sep 1 2007, 05:07 PM)

thanx,
i have that reference but there they had used its lactone as the standard for quntification, the isolation itself is a time taking procedure, i am suspicious whether can i do this with any salt of hca

-Asish.G.R-