How to get the promoter sequence of a gene - (Mar/06/2004 )

[COLOR=purple] Hello everybody!
I am an Italian student and I'm starting to learn something about bioinformatics and molecular biology!
I have a big problem and I don't know how find a solution!
I have to find the complete genome sequence of the TAF9 and TAF9L genes, I tried to find them using ncbi site, but ( I don't know why) I was not able to find nothing except the sequence starting from the point in which transcription starts, but I need what is before this point because I have to find the promoter in this region.... No comment!!!!!
I am in dangerous if I will not find them, so if someone could help me, pleaseeeeeeeeeee tell me how to do!!!!
Thanks a lot
Dada

Hi Dada,

To get the genome sequence of your genes, you can start from NCBI's loculink:

TAF9:
http://www.ncbi.nlm.nih.gov/LocusLink/LocRpt.cgi?l=6880

TAF9L
http://www.ncbi.nlm.nih.gov/LocusLink/LocRpt.cgi?l=51616

Or go to UCSC genome database:
http://genome.ucsc.edu/cgi-bin/hgTracks?or...6&Submit=Submit

Specificly, here is TAF9's genomic sequence
http://genome.ucsc.edu/cgi-bin/hgc?hgsid=3...&submit=Get+DNA

TAF9L's genome sequence
http://genome.ucsc.edu/cgi-bin/hgc?hgsid=3...&submit=Get+DNA

You can also go to Ensembl ( http://www.ensembl.org )and search by your gene name, after you get your gene, use the sequence export feature to get the genomic sequence or upstream promoter sequences.

If you have further question, please post here.

SAGE

Hi
Thanks
Very usefull information

hi
but how can i find the genome sequence of phosphoglycerate dehydrogenase gene for frog,rat ,cat ,oriza sativa ..
in the ncbi entrez page how can i find
how can i find in the ensembl
plz explain clearly
thanks

I suggest u first get the enzyme's coding sequence of rat by searching in ensembl(www.ensembl.org),then u can blast on ncbi with this sequence,I think you can get then the homolog sequence of this enzyme(if there is) in these specieses.