Cryotank storage - problem to store eukaryotes and E. coli together? (Jul/10/2007 )

is it a problem to store eukaryotic cell lines and E. coli together in one crytank?

we have a direction to store them at separate places but I wonder if there is good reason for it...

(I know mycoplasm may get out of a closed cryotube even in liquid N2 but E. coli...?)

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Dear " The Bearer ",

Good Laboratory Practice should be practiced by all, but it can lead to extra expense. For example:

Frozen Tissues should be stored seperately to cell lines.......1 cryostore

Bacteria should be stored seperately to cell lines.............1 cryostore

Primary cells should not be stored with cell lines...........1 cryostore

Therefore 3 cryostores, triple the expenditure. What other adventious agents are there that we do not know about ? Quarantining is the only way to go.


All cells, both lines and primaries should be regularly mycoplasma tested.

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so the main reason is to prevent possible contamination infection with mycoplasm? other good reasons? virus?

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The main concern for our lab would be mycoplasma contamination. We had it once, and it seemed as if it would never go.

We havnt had any other problems like virus contamination.

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I'd always thought that a -70C was good enough for E. coli glycerol stocks. Then you could keep your liquid nitrogen soley for cells.

Ceri

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