how to investigate into the mechanism? - (Jul/08/2007 )
hi,
i am testing a compound anticancer effect on ht29. my supervisor asked me to investigate into the molecular level. i plan to do mtt, flow with pi, tunel. but those are just the screening assay, anyone know how can we investigate the molecular mechanism that confers it the anticancer property?
-HKL-
QUOTE (HKL @ Jul 9 2007, 07:08 AM)
hi,
i am testing a compound anticancer effect on ht29. my supervisor asked me to investigate into the molecular level. i plan to do mtt, flow with pi, tunel. but those are just the screening assay, anyone know how can we investigate the molecular mechanism that confers it the anticancer property?
i am testing a compound anticancer effect on ht29. my supervisor asked me to investigate into the molecular level. i plan to do mtt, flow with pi, tunel. but those are just the screening assay, anyone know how can we investigate the molecular mechanism that confers it the anticancer property?
if the compound is chemically defined, I would screen the potential targets and what is known about their molecular function in cells; in parallel you should check additional cancer as well as "normal" cells;
as I know this should be also the task of your supervisor to provide some ideas...
-The Bearer-