3D cultures - collagen or matrigel? (Jul/05/2007 )
Hi, this is probably a very basic question.
I wonder about the differences between 3D cultures in matrigel and 3D cultures in collagen. Can I just use collagen instead (it is much cheaper) and will collagen affect my cells differently compared to matrigel?
-DLY-
QUOTE (DLY @ Jul 5 2007, 03:55 PM)
Hi, this is probably a very basic question.
I wonder about the differences between 3D cultures in matrigel and 3D cultures in collagen. Can I just use collagen instead (it is much cheaper) and will collagen affect my cells differently compared to matrigel?
I wonder about the differences between 3D cultures in matrigel and 3D cultures in collagen. Can I just use collagen instead (it is much cheaper) and will collagen affect my cells differently compared to matrigel?
matrigel is a mixture of various extracellular matrix proteins comprising collagen, and, moreover, matrigel contains some growth factors; matrigel is useful, for instance, to culture difficult adherent cells;
if your cells accept collagen a ECM, use it as it is really cheaper; if you like to compare your data with matrigel-derived data, you also should use matrigel...
-The Bearer-
QUOTE (DLY @ Jul 5 2007, 05:55 AM)
Hi, this is probably a very basic question.
I wonder about the differences between 3D cultures in matrigel and 3D cultures in collagen. Can I just use collagen instead (it is much cheaper) and will collagen affect my cells differently compared to matrigel?
I wonder about the differences between 3D cultures in matrigel and 3D cultures in collagen. Can I just use collagen instead (it is much cheaper) and will collagen affect my cells differently compared to matrigel?
Matrigel also induce cell differentiation. So when this process start and if you exp time is not short enough may be it is possible to see another cell structure architecture between this two coat matrixes. Also matrigel is more "native" matrix for cells in according to ECM protein content and cytokine profile, so it is also possible have influence on difference in cell organisation between Matrigel and Collagen I type matrix especially for primary cultures.
-circlepoint-
As Matrigel contains a lot of what is found in the natural ECM, you could suppose that you'd see cellular activity that more closely resembles what would be found naturally. Collagen is cheaper and it's easier to wirk with. An alternative to both of these that sort of falls in the middle is Extracel, made by Glycosan Biosystems www.glycosan.com.
-Biolurp-
I was wondering if anyone has a protocol for staining cells in 3-D collagen with periodic acid Schiff?
Thank you.
-YANGmeister-
QUOTE (Biolurp @ Aug 21 2007, 11:00 PM)
As Matrigel contains a lot of what is found in the natural ECM, you could suppose that you'd see cellular activity that more closely resembles what would be found naturally. Collagen is cheaper and it's easier to wirk with. An alternative to both of these that sort of falls in the middle is Extracel, made by Glycosan Biosystems www.glycosan.com.
Did you try this Extracel?If so, what can you say about the effect?
-Roman80-
QUOTE (Roman80 @ Apr 15 2008, 11:31 AM)
QUOTE (Biolurp @ Aug 21 2007, 11:00 PM)
As Matrigel contains a lot of what is found in the natural ECM, you could suppose that you'd see cellular activity that more closely resembles what would be found naturally. Collagen is cheaper and it's easier to wirk with. An alternative to both of these that sort of falls in the middle is Extracel, made by Glycosan Biosystems www.glycosan.com.
Did you try this Extracel?If so, what can you say about the effect?
I work with the hyaluronic acid material from glycosan (PEGDA, gelatin, HA), I think they have some other polymers, too. Matrigel (I believe) is derived from ECM of cancer cells, and its composition also varies from batch to batch, and you are never sure what is in there. Matrigel is good to use as a control, to say what cells should be doing. With collagen, you are just giving the cells on ECM protein to work with, and sometimes that is enough. I grow endothelial progenitor cells on collagen, and they proliferate and grow/attach on collagen (only way they can grow), but other cells may express different behavior when grown on collagen.
-eli2k-