lysis buffer- protease inhibitors - (Jun/26/2007 )

Hi all,
I don’t have much experience with apoptosis.

I should assess caspase-9 activity using a glo-type lumiscent kit.

This is the composition of the Lysis buffer that I am going to use:
20 mM Hepes-KOH, pH 7.5, 250 Mm saccharose; 50 mM KCl; 2.5 mM MgCl2; 1Mm Dithiothreitol. Does it need to be supplemented with protease inhibitors?

Any tips or suggestions will be very much appreciated.

Thanks