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what contribute the two emission peaks of CFP individually - the molecular mechanism of the two emssion peaks of CFP (Jun/20/2007 )

you know that the Cyan Fluorescent protein have two emission peaks, does anybody know that what group contribute to them individually?Thank you in advance!

-subberry-

Hello Subberry,

I can only try to guess...

CFP has a Trp66 instead of Tyr66 (GFP).
The spectra of Trp has different peaks, too (see attachment). They are dependent of the aromatic stages of Trp.


Maybe this is the reason? huh.gif

Greetings,
Chakchel

P.S.: Maybe "Heim, R., Prasher, D. C., Tsien, R. Y., Proc. Natl. Acad. Sci. USA (1994) 91, 12501-12504" gives you some more suggestions - it describes different mutants of GFP

-Chakchel-

THank you,Chakchel!your expalin sounds reasonable!


QUOTE (Chakchel @ Jun 20 2007, 09:37 PM)
Hello Subberry,

I can only try to guess...

CFP has a Trp66 instead of Tyr66 (GFP).
The spectra of Trp has different peaks, too (see attachment). They are dependent of the aromatic stages of Trp.


Maybe this is the reason? huh.gif

Greetings,
Chakchel

P.S.: Maybe "Heim, R., Prasher, D. C., Tsien, R. Y., Proc. Natl. Acad. Sci. USA (1994) 91, 12501-12504" gives you some more suggestions - it describes different mutants of GFP

-subberry-