RNA extraction problem - (Jun/13/2007 )

Hi all,

I have extracted RNA from flower (petal and pistil tissues) with TRizol protocol.
I have done the additional step (a centrifugation for 10min at 4°C) for samples with high content of protein and polysasaccharides as my tissue. Well for the petals all is ok, the 260/280 ratio were 1,9, but for the pistil I have a terrible 260/280 ratio approximately 0.8 and the RNA certainly is degradated. How can I do to increase the purification to obtain a plausible ratio?

Could you help me?

Thank you in advance

Alice

-sisma-

QUOTE (sisma @ Jun 13 2007, 11:35 AM)

Hi sisma,
The 260/280 ratio may not mean much in RNA purity.

http://en.wikipedia.org/wiki/Quantification_of_nucleic_acids

-WHR-

Have you tried to run your sample under agarose?

Have you use any protocol to avoid RNase degradation? like using DEPC, using filter tips etc?

The ratio wouldnt mean much but at least you will slightly know if there is RNA with 1.9 value. Good luck!

-timjim-

QUOTE (timjim @ Jun 17 2007, 05:23 PM)

Have you tried to run your sample under agarose?

Yes I have tried and the RNA samples with lower ratio, were degradated

Have you use any protocol to avoid RNase degradation? like using DEPC, using filter tips e

The ratio wouldnt mean much but at least you will slightly know if there is RNA with 1.9 value. Good luck!

-sisma-