microarrays with alexa fluors instead of CyDyes - microarrays with alexa fluors instead of (Dec/09/2003 )

has anyone tried doing microarrays using amino-allyl dUTP and alexafluor dyes (Molecular Probes) rather than CyDyes? I have been having major problems with Cy5 degradation (when I scan i can't see much/any Cy5) and would really appreciate any advice.

-ros-

what do you wash with i had the same problem (amersham dyes) and i replced my ethanol wash with low salt and know problems anymore regarding the signal

-DML-

What do you mean washed with low salt? Could you be more specific?

Thanks

-kdandrea-

Please find the citation from the following link.
http://www.ncbi.nlm.nih.gov/entrez/query.f...nfft&query_hl=8

Quantitative Comparison of Long-wavelength Alexa Fluor Dyes
to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates
Volume 51(12): 1699–1712, 2003
The Journal of Histochemistry & Cytochemistry
http://www.jhc.org

There are several citations state that Alexa Fluor Dyes have a higher stability, better correlation, higher incorporation efficiency and higher intensity than Cy dyes.

-sallylyc-