RANDOM PCR PROTOCOL? - Looking but no finding... (May/31/2007 )
Hellow, I´d like to amplify DNA by random PCR using random hexamers. Does anyone know how to do this? I´m looking for protocols but I just find kits or reactions for labeling or amplify cDNA. Thaks so much. 
-Mnemonia-
QUOTE (Mnemonia @ May 31 2007, 08:46 AM)
Hellow, I´d like to amplify DNA by random PCR using random hexamers. Does anyone know how to do this? I´m looking for protocols but I just find kits or reactions for labeling or amplify cDNA. Thaks so much.
Why don't you try with protocols for RAPD but primers for this purpose ussually are 10mers that anneals at 30-40 oC. or lower
-Doyo-