List of common wavelengths used in Molecular Biology and Immunology - (May/23/2007 )

Hi, Folks!

Does anyone have a list of common wavelenghts (absorbance; UV and visible) used in Molecular Biology and Immunology assays? I mean, a table with some information like 260nm (UV) -> DNA/RNA; 595nm -> Proteins (Bradford); 405nm, 450nm, 490nm, etc. -> ELISA...

I need this because we are about to buy our first microplate reader and I would like to include in my order some extra filters that may be useful in the future. Many thanks in advance!

Best regards,

DEO

-Elgui-

QUOTE (Elgui @ May 24 2007, 06:44 AM)

You could add 214 or 256 for proteins (peptide bond or aromatic residues), 280 for proteins in DNA/RNA preps (to get a 260/280 ratio), 620 for ELISA

-swanny-

QUOTE (Elgui @ May 23 2007, 09:44 PM)

for double wave length measuring you need always a reference; so 620-630 nm as reference for 405-490 nm should work in many cases; if you have more money, think of a fluorescence light plate reader

-The Bearer-

550->biuret protein determination;

500,650,700,750-> lowry protein determination (depending on desired sensitivity);

660-> fiske-subbarow phosphate determination;

340-> nad(p)h;

820-> chen phosphate determination;

310-> marsh phosphate determination;

it boils down to: you have to determine what you are going to use it for, now and in the foreseeable future, and then order what you need. anything else can be ordered later.

-mdfenko-