dam minus host strain - (May/21/2007 )

can anybody tell me which strains are Dam plus and which are Dam minus strain
specifically Top10 cells and subcloning effeciency Dh5A cells
thanks

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i use E. coli INV110

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Have you checked the New England Biolabs catalogue? It usually has heaps of that kind of information.

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Yeah, NEB always has good info about this. I use GM2163 from NEB, but I think that they have newer strains for dam- now.

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Wondering the same thing. Looked at neb web-catalogue but did not find what you talk about (is it a list with all + and - strains?)

The strain I wonder about is from invitrogen (Mach1). I have looked at the product sheet but do not find anything about it...

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err no. You have a list of strains (also in promgea or other webstes) and for each genotype is described.

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One more hint...? (I'll go crazy, I do not find my bacteria!)

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Hello Ammie,

some common strains' genotypes are also listed in wikipedia:

http://openwetware.org/wiki/E._coli_genotypes

There you can find a short and nice description of the nomenclature, too.

Mach1 from Invitrogen: ΔrecA1398 endA1 tonA Φ80ΔlacM15 ΔlacX74 hsdR(rK- mK+)

On the website of invitrogen you can also find the genotypes in the manuals (if you don't have a printed one).

Greetings,
Chakchel

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Thanks Chrackel!

I found that information also but I guess that the problem is that I do not really know how to interpret it... So if one property is not mensioned, that means that it is the wildtype? (dam+ for mach1 for example)?

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That's right Ammie.

Only the mutant alleles are mentioned in the genotype. More info here if required.

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