autoclave PCR tubes... - (May/16/2007 )

Does anyone know.....When autoclaving PCR tubes and microcentrifuge tubes, is it best to shut the lids or leave them open (when in a sealed container)?
Thanks!

Does anyone know.....When autoclaving PCR tubes and microcentrifuge tubes, is it best to shut the lids or leave them open (when in a sealed container)?
Thanks!

they are sold DNase and RNase free ,you don't need to autoclave them..

I thought I was the only one who thought that way...everyone else I know autoclaves them. My thinking has always been that they're already sterile and in a sealed bag, and what if there is something wrong with the autoclave that you don't know about?

An autoclave method for preparing bacterial DNA for PCR template is presented, it eliminates the use of detergents, organic solvents, and mechanical cellular disruption approaches, thereby significantly reducing processing time and costs while increasing reproducibility. Bacteria are lysed by rapid heating and depressurization in an autoclave. The lysate, cleared by microcentrifugation, was either used directly in the PCR reaction, or concentrated by ultrafiltration. This approach was compared with seven established methods of DNA template preparation from four bacterial sources which included boiling Triton X-100 and SDS, bead beating, lysozyme/proteinase K, and CTAB lysis method components. Bacteria examined were Enterococcus and Escherichia coli, a natural marine bacterial community and an Antarctic cyanobacterial-mat. DNAs were tested for their suitability as PCR templates by repetitive element random amplified polymorphic DNA (RAPD) and denaturing gradient gel electrophoresis (DGGE) analysis. The autoclave method produced PCR amplifiable template comparable or superior to the other methods, with greater reproducibility, much shorter processing time, and at a significantly lower cost.