Culture of rat cortical neurons - E16 or E18? (May/15/2007 )
Thanks!
Saya, the paper is very complete, really good 
Finally, yesterday we had a discussion in our lab: some people said that the pregnant rat should be very quickly killed by cervical dislocation or guillotine without being anesthetized 
(I suppose this wouldn´t be approved by an ethical comittee...) because anesthesia would damage embryonic neurons and reduce viability, while others said that it must be killed by an overdose of anesthesia.
Furthermore, in the case of anesthesia, they also didn´t agree because some people thinks that i.p. anesthesia (pentobarbital) causes more damage to the embryos (or, at least, is quicker) that the inhaled one (halothane).
what do you think? 
Saya, the paper is very complete, really good
Finally, yesterday we had a discussion in our lab: some people said that the pregnant rat should be very quickly killed by cervical dislocation or guillotine without being anesthetized
(I suppose this wouldn´t be approved by an ethical comittee...) because anesthesia would damage embryonic neurons and reduce viability, while others said that it must be killed by an overdose of anesthesia. Furthermore, in the case of anesthesia, they also didn´t agree because some people thinks that i.p. anesthesia (pentobarbital) causes more damage to the embryos (or, at least, is quicker) that the inhaled one (halothane).
what do you think?
We anesthesize pregnant rats by CO2. If rats remained more in CO2 chamber it can kills the rat because of deep anesthesia and therefore no cervical dislocation is needed. Personally I prefer to anesthesize the rat with CO2 and then do cervical dislocation. It workes fine.
That´s a good option. thank you
We used to kill animals by CO2 in my old lab. Now, we just kill it by isofluorane and then by cervical dislocation before opening the abdomen.
ok,so it seems there is no problem for neurons if you kill with anesthesia.
Thanks scolix