Protein lysis buffer for mass spec - recipe (May/11/2007 )
Forgot about PBS. Excuse me.
So what protease inhibitor did you use?
-timjim-
QUOTE (timjim @ May 15 2007, 07:28 AM)
Forgot about PBS. Excuse me.
So what protease inhibitor did you use?
So what protease inhibitor did you use?
Nevermind! I am trying to find out which protease inhibitor we are using...it's aliquoted in unlabeled vials and a no one knows where it comes from! I JUST LOVE THOSE PEOPLE! 
-Jou2007-
Hmmm.. Unlabeled vial? Yea... the people are just so lovable.
-timjim-
You might think about adding EDTA to the mix.
-phage434-
EDTA interferes with mass spec analysis... 
-Jou2007-
Im no specialist, but isn´t it possible to dialyze against the desired buffer, or usage of gelfiltration technique before MS?
-moljul-