Polymerase for Long PCR - (Apr/24/2007 )

Can some body suggest me the polymerase for amplification of gene for 123 kb size

-samita-

QUOTE (samita @ Apr 24 2007, 02:05 PM)

Can some body suggest me the polymerase for amplification of gene for 123 kb size

try platinum taq by invitrogen, with or without proof reading........ good luck

-mode_vigilante-

123 kb??? do you mean 12,3?

If so, platinum taq should indeed do it, as should expand long template from roche, colleages of mine are succesful with PfuultraIIHS from stratagene.

-vairus-

123 kb PCR product!
OMG! If anybody know how to make a PCR product that long I humbly beg for your infinite wisdom.

If 12.3kb, another enzyme to add to the list is Phusion from Finzyme

-perneseblue-

I think bp in the gene is 3362. If i am not wrong then its protein size should be.
3362/3 = 1120 * 110 /1000 = 123.2 I think the gene is 123.2 kb. Am i right or wrong. please correct me.
regards

QUOTE (perneseblue @ Apr 24 2007, 02:35 PM)

123 kb PCR product!

OMG! If anybody know how to make a PCR product that long I humbly beg for your infinite wisdom.

If 12.3kb, another enzyme to add to the list is Phusion from Finzyme

-samita-

If your gene is 3362 bases, then it's 3,362 kilo-bases or kb. (kb means 1000 bases).

-vairus-