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Genomic DNA hybridization for species identification - (Apr/22/2007 )

Hi guys,

I need some quick method, non radioactive if possible, to make a genomic DNA hybridization. The idea is to obtain genomic DNA from two different strains and to know quickly how much homology they share. This is the classical Cot experiment, but I would like to do it without radioactivity if it is possible. There are kits for everything today, but I can't find something for this.

Many thanks!

Cheers.

-Biopolako-

That's because very few people do this any more. The measurement is pretty crude, and the results are not very definitive. Here in the future, most people choose a few standard genes and sequence them. I do think it would be pretty easy to substitute fluorescence for radioactivity if you really want to do it.

-phage434-

agree with phage434, try doing fluorescence

-strawberry-