Asimetric PCR - primer relation (Oct/07/2003 )

Hi,

I am tryng to obtain ss PCR products to use as megaprimers. Does anyone knows a protocol?, since primers relation 1:100 that I have read gives me faint bands.

Thank you!!!!

Use one phosphorylated primer and one non-phospho primer. PCR normally add Lambda nuclease. Posphorylated strand is digested.

good luck