shRNA Method - (Apr/18/2007 )

does anybody have an idiot proff guide to using shRNA for the first time? is it just a case of ordering the vector, transfecting it in and bob's your uncle?

cheers

Look for reviews and go thru them.

You could order a complimentary book from Dharmacon abt RNAi.
The website provides a recommended reading list besides other important information.