DNA Extraction-pigment contamination - (Apr/14/2007 )
I extracted DNA from plant shoot meristem tissue. However, the DNA that I got is reddish brown in color (anthocyanin contamination?) I used the CTAB method and repeated the wash buffer step several times to get rid of the color...didn't work :-(
Can anyone suggest some way to get rid of the color?
-SonaliM-
Why do you care? Often colored material can be removed with carbon black followed by filtration.
-phage434-
Wouldn't it affect PCR and Southern hybridisation?
-SonaliM-
Probably not. Try it and see.
-phage434-
Well my PCR didn't work today :-((
-SonaliM-
Is there a control reaction which is working? Do you know the primers and annealing temperature are working? Often the problem is too much template DNA. How much are you adding?
-phage434-
QUOTE (SonaliM @ Apr 14 2007, 11:20 PM)
I extracted DNA from plant shoot meristem tissue. However, the DNA that I got is reddish brown in color (anthocyanin contamination?) I used the CTAB method and repeated the wash buffer step several times to get rid of the color...didn't work :-(
Can anyone suggest some way to get rid of the color?
Can anyone suggest some way to get rid of the color?
There was already a thread about this, look here
-hobglobin-