CFP-protein - fuses proteins (Apr/14/2007 )
Hi
If I crea a fusion protein by potine the protein A in C terminal in the CFP vector (CFP-A) should I remove the ATG of my protein?
is it critical because the transcription could start from the CFP ATG or from the A ATG. in this cae I may get my full lenght fused protein and only the CFP.
any advice?
ulujm
-ulujm-
QUOTE (ulujm @ Apr 14 2007, 11:21 AM)
Hi
If I crea a fusion protein by potine the protein A in C terminal in the CFP vector (CFP-A) should I remove the ATG of my protein?
is it critical because the transcription could start from the CFP ATG or from the A ATG. in this cae I may get my full lenght fused protein and only the CFP.
any advice?
ulujm
If I crea a fusion protein by potine the protein A in C terminal in the CFP vector (CFP-A) should I remove the ATG of my protein?
is it critical because the transcription could start from the CFP ATG or from the A ATG. in this cae I may get my full lenght fused protein and only the CFP.
any advice?
ulujm
Which vector are you using?
-Cassio-