MacConkey agar - used to identify G-ve ? (Apr/13/2007 )
is MacConkey agar used as a tool to identify G-ve bactera?
does ALL G-ve bacteria grow on it? do ANY G+ve ones grow on it?
-ahmad193-
Mac conkey agar is used :
1) 2 differentiate Gram-negative enteric bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-).
2) 2 identify Gram negative enteric pathogens
3) Bile salt & Crystal Violet (key ingredient of Mac conkey agar) - inhibits de growth of Gram positive bacteria
Not all Gram negative bacteria can grow on Mac conkey agar such as Campylobacter, helicobacter, Haemophilus etc, only those belonging 2 the
family : Enterobacteriaceae can grow on them
-dhanu_609-
QUOTE (dhanu_609 @ Apr 16 2007, 01:07 PM)
Mac conkey agar is used :
1) 2 differentiate Gram-negative enteric bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-).
2) 2 identify Gram negative enteric pathogens
3) Bile salt & Crystal Violet (key ingredient of Mac conkey agar) - inhibits de growth of Gram positive bacteria
Not all Gram negative bacteria can grow on Mac conkey agar such as Campylobacter, helicobacter, Haemophilus etc, only those belonging 2 the
family : Enterobacteriaceae can grow on them
1) 2 differentiate Gram-negative enteric bacteria that can ferment the sugar lactose (Lac+) from those that cannot (Lac-).
2) 2 identify Gram negative enteric pathogens
3) Bile salt & Crystal Violet (key ingredient of Mac conkey agar) - inhibits de growth of Gram positive bacteria
Not all Gram negative bacteria can grow on Mac conkey agar such as Campylobacter, helicobacter, Haemophilus etc, only those belonging 2 the
family : Enterobacteriaceae can grow on them
merci beaucoup
-ahmad193-