PCR protocols - for some antibiotic resistant strains (Apr/12/2007 )
Hey,
I was wondering if anyone has the PCR protocols for these strains of bacteria: Staphylococcus aureus, Enterococcus spp, Acinetobacter baumannii, Klebsiella spp
plz post any info on identification primers and also antibiotic resistant genes/primers available
you'd really make my life a lot easier! 
-ahmad193-
if you want resistance gene primers, the best place to go is www.pubmed.com and do a literature search
likewise with pcr conditions. it will be different for each set of primers, and is not organism-specific. the only thing that will change between one bacterium and the next, is the DNA extraction protocol.
-aimikins-
QUOTE (aimikins @ Apr 12 2007, 04:34 PM)
if you want resistance gene primers, the best place to go is www.pubmed.com and do a literature search
likewise with pcr conditions. it will be different for each set of primers, and is not organism-specific. the only thing that will change between one bacterium and the next, is the DNA extraction protocol.
likewise with pcr conditions. it will be different for each set of primers, and is not organism-specific. the only thing that will change between one bacterium and the next, is the DNA extraction protocol.
thank you amigo.. i'll do just that and get back at u
-ahmad193-