About TF and DNA - Do they affect both strands? (Mar/27/2007 )
Hi,
I have to study the promoter of a gene and the effects of Transcription factors on it.
About them do I have to take into account the TF that are found on both strands? I mean, I did some bioinformatic analysis and predicted TF sites on the + and - strand, if the transcript is from the - strand, do I care about TF that bind to the + strand??
thank you
biotech
-biotech!-
QUOTE (biotech! @ Mar 27 2007, 05:07 PM)
Hi,
I have to study the promoter of a gene and the effects of Transcription factors on it.
About them do I have to take into account the TF that are found on both strands? I mean, I did some bioinformatic analysis and predicted TF sites on the + and - strand, if the transcript is from the - strand, do I care about TF that bind to the + strand??
thank you
biotech
I have to study the promoter of a gene and the effects of Transcription factors on it.
About them do I have to take into account the TF that are found on both strands? I mean, I did some bioinformatic analysis and predicted TF sites on the + and - strand, if the transcript is from the - strand, do I care about TF that bind to the + strand??
thank you
biotech
I believe you do need to take into consideration of both + and - strands. Considering that TF might help to open DNA double strand transiently by forming a complex, they could potentially bind on either or both strands.
-cyberpostdoc-