how to detach the albumin on PET - SOS (Mar/22/2007 )

hola everyone

Do you know which solvent I can use to detached albumine on PET surface. Because with Plasmon (it's a machine which can see interaction between proteines and biomaterials. I put albumine and normally with Isopropanol, albumine will be detached but not in our case..

thanks A LOT

THAT IS IN A HURRY

-lilylille-

PET is hydrophobic, therefore a detergent, such as SDS, may help getting it off.

-genehunter-1-

thanks for quick anwser.

But SDS is biologicallly safe or not? For afterwards we need this substrate for cell culture test

some colleagues propose something like Twin 20. I think it is a non ionic surfactant. it is good or not??

thanks again

QUOTE (genehunter-1 @ Mar 22 2007, 07:10 AM)

PET is hydrophobic, therefore a detergent, such as SDS, may help getting it off.

-lilylille-

some colleagues propose something like Twin 20. I think it is a non ionic surfactant. it is good or not??

-lilylille-

Its hard to predict. Why dont you let your results to lead your next move? Cells dont attach to PET readily if PET is not surface treated.

-genehunter-1-