Post-chromosomal supernatant - what is it?! - (Mar/19/2007 )

I've found this expression in several papers but didn't find an exact definition. Can anyone help me what does post-chromosomal supernatant means? How it has to be prepared? What kind of cell components it contains?
Thanks a lot!
J.

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I think it is the same as the also often used "postnuclear" supernatant which means the supernatant after only pelleting the nuclei (which bear the chromosomes) during subcellular fractionation

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uhh, so only the chromosomes are missing from the cell lysate?

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normally you do it by differential centrifugation; may be some larger debris are co-pelletd with nuclei; but during preparation nuclei should be intact; if they are lysed or homogeneized, simple non-gradient centrigugation to fractionize becomes difficult...

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sorry, I'm really not in this fiedl but I want to know: what components can be found in a postnuclear supernatant, and what goes to the pellet?
cheers

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you can pellet in density gradient and non-gradient centrifugation; postnuclear supernatant can be achieved by at least 1000xg for 10 min in a non-gradient centrifugation; in the postnuclear supernatant remain mitochondria, lysosomes, microbodies, various membrane vesicles and cytoplasm; the higher the g-force the less chromosomes and other organelles you have

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Now I think it is clear. Thanks!
Judit

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