lysing yeast - (Mar/13/2007 )
Can anybody tell how to lyse the yeast cells. I also like to know if zymolyase or lyticase is effective in lysing the cells. Ahich one is better?
-sktewary-
Yes, either enzyme will lyse yeast, and seem to be equivalent in our experience. You can also use a bead-beater and glass beads for mechanical disruption.
-phage434-
QUOTE (phage434 @ Mar 13 2007, 02:37 PM)
Yes, either enzyme will lyse yeast, and seem to be equivalent in our experience. You can also use a bead-beater and glass beads for mechanical disruption.
I agree. You don't see much difference. Although my lab currently uses zymolyase.
-perneseblue-