Protocol Online logo
Top : Forum Archives: : Molecular Biology

PCR Cloning - (Jul/13/2003 )

I try to clone ~800bp in home made TA vector PUC19. My question is Does dephosphorelation after the linearization by SmaI is important for successful recombination?

-Magdy-

just give it a try!!!!!!!!!!!!!!!!

-bobprashanth-