What is the difference between HBSS and PBS? - (Mar/12/2007 )

Hi, all
I am so excited to find such a helpful website!

Could anyone please tell me what is the difference between HBSS and PBS? Can they be replaced with each other in most conditions?

Thanks a lot!

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Google: "HBSS PBS recipe" :

HBSS
400 ml H₂O

4.0 g NaCl (=13.7 ml 5 M NaCl)
0.20 g KCl
0.50 g Glucose
0.021 g Na₂HPO₄
0.027 g KH₂PO₄
0.096 g CaCl₂
0.111 g MgCl₂
0.176 g NaHCO₃

qc 500 ml
pH 7.1
Filter sterilize

PBS
900 ml H₂O
8 g NaCl or 27.4 ml 5M
0.2 g KCl
0.19 g KH₂PO₄ or 1.4 ml 1M
0.61 g Na₂HPO₄ or 4.3 ml 1M

qc 1 L
pH 7.4

autoclavable

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thanks!

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