DNA ends after sonication - Blunt ends or not? (Feb/20/2007 )

Hi all!
I´m trying to perform a clonation with DNA fragments after ChIP, but I don´t know if broken DNA ends are blunt or not. If not, maybe its better to amplify by ramdom primer pcr.
Does anyone know?
Thanks

As far as I know you will have a mix of 5', 3' and blunt ends after sonication.

Maybe try a restriction digest?

Goofy

Something like this might also help you.

http://www.talron.co.il/index.php?module=p..._position=86:83

Just an example! I'm sure there are competing products from other companies as well!

Goofy

If you're trying to clone, do you have an idea of a particular target gene? If so, then PCR is the best way to go. If you just want to clone everything, and decide later which clones to use, I'd try blunt-ending the DNA, rather than doing a restriction digest, and cloning the blunt-ended fragments.

Thanks you all. I want to clone all my sheared productos, and then identify them. I was thinking to treat my sheared DNA with T4 DNA polimerase or Klenow to complet posible extrems, then clone this in a vector, and finally PCR and sequencing.
Thanks!!!