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Difference between terms Vitality, Viability, Growth and Proliferation - (Feb/16/2007 )

hi everyone. who could be so kind to make me clear about those terms about the cell culture VITALITY, VIABILITY, or PROLIFERATION. I really experienced different period with them, feel not understand firstly, then understand better, then again feel not understand well. That comes from the routine manip in our lab, we do cell culture in 24 well plate and after 3 or 6 days, we test vitality (at least I was taught like this) with Alamarblue, because cell still maintain in good form, then we detacth cell with Trypsin and counting cell number with Cell Counter (here I was told the results from cell counting could be considered as proliferation). But actually after reading the booklet of Alamarblue, this product is considered to be used to determine the cell proliferation, so that means in our lab we did those two steps perhaps not necessary, for they reflect the same aspect of cell culture. that is my own opinion. but not clear at all, please if somebody are clearer, could you make it clear for me?

thanks

-lilylille-

Cell viability is the determination of living or dead cells from a total cell count. For example, after trypsinization and staining with trypan blue, count the total number of cells. Then count the number of live cells, and divide that number by the total cell count, mutliply by 100. That will give you a % cell viability.

The same technique can be applied for cell proliferation, which is the same thing as cell division. To calculate the rate of division, count the live cells on Day 1. Then count again on Day 2. Divide the count on day 2 by the count on Day 1. That will give you the "rate" of proliferation, or rate of division over 24 hrs. HOpe that helps!

-Dubs-

QUOTE (lilylille @ Feb 16 2007, 08:42 AM)
hi everyone. who could be so kind to make me clear about those terms about the cell culture VITALITY, VIABILITY, or PROLIFERATION. I really experienced different period with them, feel not understand firstly, then understand better, then again feel not understand well. That comes from the routine manip in our lab, we do cell culture in 24 well plate and after 3 or 6 days, we test vitality (at least I was taught like this) with Alamarblue, because cell still maintain in good form, then we detacth cell with Trypsin and counting cell number with Cell Counter (here I was told the results from cell counting could be considered as proliferation). But actually after reading the booklet of Alamarblue, this product is considered to be used to determine the cell proliferation, so that means in our lab we did those two steps perhaps not necessary, for they reflect the same aspect of cell culture. that is my own opinion. but not clear at all, please if somebody are clearer, could you make it clear for me?

thanks


May be I will try to begin

First of All Viability and vitality are equal they mean live energy of your cells. For ex standart method to estimate cell viability is Tripan blue staining. If the membrane of cell is damages then this colored substance permeable into cytoplasm and stain intracellular proteins. I always combine this staining procedure with counting cells in Hemacytometer. (10mkl cell suspension + 10 mkl 0,1% Tripan blue in PBS. Died or damaged cells stained blue.



About difference between using dyes for testing viability and proliferation I think following : the dyes for estimation viability - only permeable into cytoplasm ( if cell membrane damaged) and bind with intracellular proteins without formation of new coloured substance. So we can't determine cell proliferation because only damaged cells only stained and others viable cells are not stained. When we use Dyes for estimation of proliferation, These substances are substrates for enzymes, which are in cells catalyze convertion them into colored substances. So production of these colored products will be in direct proportion with activity of these enzymes. The extent of enzyme activity depends on cell activity which may be send for proliferation. By the way see the following paper http://content.febsjournal.org/cgi/content/full/267/17/5421 here describes disadvantages of AlamarBlue for proliferation\cytotoxic assay.

Another interesting material may be :

http://www.manfred.maitz-online.de/Publications/Presentations/Lecture2005/Lesson07.pdf

-circlepoint-

QUOTE (circlepoint @ Feb 16 2007, 11:46 AM)
QUOTE (lilylille @ Feb 16 2007, 08:42 AM)
hi everyone. who could be so kind to make me clear about those terms about the cell culture VITALITY, VIABILITY, or PROLIFERATION. I really experienced different period with them, feel not understand firstly, then understand better, then again feel not understand well. That comes from the routine manip in our lab, we do cell culture in 24 well plate and after 3 or 6 days, we test vitality (at least I was taught like this) with Alamarblue, because cell still maintain in good form, then we detacth cell with Trypsin and counting cell number with Cell Counter (here I was told the results from cell counting could be considered as proliferation). But actually after reading the booklet of Alamarblue, this product is considered to be used to determine the cell proliferation, so that means in our lab we did those two steps perhaps not necessary, for they reflect the same aspect of cell culture. that is my own opinion. but not clear at all, please if somebody are clearer, could you make it clear for me?

thanks


May be I will try to begin

First of All Viability and vitality are equal they mean live energy of your cells. For ex standart method to estimate cell viability is Tripan blue staining. If the membrane of cell is damages then this colored substance permeable into cytoplasm and stain intracellular proteins. I always combine this staining procedure with counting cells in Hemacytometer. (10mkl cell suspension + 10 mkl 0,1% Tripan blue in PBS. Died or damaged cells stained blue.



About difference between using dyes for testing viability and proliferation I think following : the dyes for estimation viability - only permeable into cytoplasm ( if cell membrane damaged) and bind with intracellular proteins without formation of new coloured substance. So we can't determine cell proliferation because only damaged cells only stained and others viable cells are not stained. When we use Dyes for estimation of proliferation, These substances are substrates for enzymes, which are in cells catalyze convertion them into colored substances. So production of these colored products will be in direct proportion with activity of these enzymes. The extent of enzyme activity depends on cell activity which may be send for proliferation. By the way see the following paper http://content.febsjournal.org/cgi/content/full/267/17/5421 here describes disadvantages of AlamarBlue for proliferation\cytotoxic assay.

Another interesting material may be :

http://www.manfred.maitz-online.de/Publications/Presentations/Lecture2005/Lesson07.pdf



Thanks a lot

especially this one http://www.manfred.maitz-online.de/Publica...05/Lesson07.pdf[/b]
I like it very much, it is quite fit my researching field, I learned something else more. Thanks agian

-lilylille-