Pseudo-gene identification - (Feb/16/2007 )

hi ppl,

I have a few ab-initio predicted protein sequences.

1. i want to know, if any of these might be a pseudo-gene. So how do i go about it?

2. Can dN/dS comparison across related sequences tell me, if its a pseudo-gene ?

i need some help on this .....

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Typically, pseudogenes lack introns. You could go to the Human Genome database, pull out the gene your think you have, select some intronic sequence and test your sequence for that.

Also, pseudogenes will have mutations that the true gene won't have, so you might have luck by aligning your predicted sequence against the consensus protein sequence.

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Thanks swanny . Hope to pick out them ......

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Great. Please let us know how they go.

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