about Filter Paper Assay - some quiry (Feb/16/2007 )
hi all
i need to start filter paper assay(FPA) soon, so i want remove some of my confusion as i will be the 1st person in the lab who will perform FPAssay
q1. after insert filter paper do i need to vortex it to mix with the enzyme soln??
q2. how you add 20 ml water in each tube?? i want to use a plastic mess cylinder. is it ok??or i need to use glass pippette
q3. do you get any differnece betn spectra zero and sample blank ?(after set the spectra zero at zero i got my blank sample reading also zero)
q4. do i need to construct new glucose standard curve every single time i perform the assay???
q5. how long you wait after add water??
ok thats all for today, advance thanks for your help
i need to start filter paper assay(FPA) soon, so i want remove some of my confusion as i will be the 1st person in the lab who will perform FPAssay
q1. after insert filter paper do i need to vortex it to mix with the enzyme soln??
q2. how you add 20 ml water in each tube?? i want to use a plastic mess cylinder. is it ok??or i need to use glass pippette
q3. do you get any differnece betn spectra zero and sample blank ?(after set the spectra zero at zero i got my blank sample reading also zero)
q4. do i need to construct new glucose standard curve every single time i perform the assay???
q5. how long you wait after add water??
ok thats all for today, advance thanks for your help
what do you like to assay? kinases?
total cellulase activity
please someone reply me................i really need some suggestionsssssss