mirVana and Parafin-embedded tssues - (Feb/15/2007 )

Hello everybody

Have anybody used the mirVana isolation kit for miRNA isolation from formalin fixed and paraffin embedded tissues and which are the step of the protocol.

Thanks

Hello everybody

Have anybody used the mirVana isolation kit for miRNA isolation from formalin fixed and paraffin embedded tissues and which are the step of the protocol.

Thanks

Just bought a kit from ambion to test it.
miRNAs are considered to be very stable so it should work. When I read the manual the only thing that is different from other miRNA isolation kits is the extra de-paraffinization step.
Mayby i'm wrong but I will find out

ari